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. 2023 Jul 24;13:11931. doi: 10.1038/s41598-023-38860-w

Figure 5.

Figure 5

Amplification profile and anneal derivative curve comparison of Varroa gBlock gene fragment (synthetic positive control) and V. destructor. (a) Amplification profile of five dilutions of V. destructor DNA (VAITC 9797a) ranging from 4 ng/µL to 1.56 × 10–2 ng/µL (amplification time 10 to 20 min) and gBlock 1 × 106 copies/µL, at 10 min (dotted pink line). Negative, no amplification (blue). (b) Anneal derivative of LAMP amplicons showing two peaks, 78 °C for V. destructor, and 80 °C for the gBlock (dotted pink line).