Table 1.
Varroa LAMP primer and amplicon sequences (gBlock) and parameters.
| LAMP primer or amplicon | Sequence 5'-3' | Primer length (bp) | Predicted Tm (°C) | Primer degeneracy (fold) |
|---|---|---|---|---|
| Varroa_F3 | CGGTTTATCCTCCTTTATCARGAAAT | 26 | 65.1 | 2 |
| Varroa_B3 | CGATCTGTTAAYAATATTGTAATAGCYCC | 29 | 64.0 | 4 |
| Varroa_FIP | AAATAGTAGCAATAAAATTAATAGATCTTATAATAGTAGAGGWGTAGCAGTTGATTTAGG | 60 | 71.4 | 2 |
| Varroa_BIP | TAAATATACGTGTWAAGGGRATAAATAAACAGGYAAAGATAATAATAATAAAATAGTAG | 59 | 70.3 | 8 |
| Varroa_Floop | ARGARATTCCAGCTAAATGYAAAC | 24 | 62.2 | 8 |
| Varroa_Bloop | GAAATAATRCCTTTATTTGTATGRTCWGTT | 30 | 62.8 | 8 |
| Varroa_gBlock fragment | cccCGGTTTATCCTCCTTTATCAGGAAATcccTAGAGGTGTAGCAGTTGATTTAGGcccGTTTGCATTTAGCTGGAATCTCCTcccCTATTATAAGATCTATTAATTTTATTGCTACTATTTcccTAAATATACGTGTAAAGGGGATAAATcccGAAATAATGCCTTTATTTGTATGGTCTGTTcccCTACTATTTTATTATTATTATCTTTGCCTGTTTTcccGGAGCTATTACAATATTGTTAACAGATCGccc | 256 | N/A |
The F2 and B2 primer regions of FIP and BIP are shown in bold and underlined. Lowercase letters in the gBlock indicate extra c’s added between LAMP primer sites to increase the overall Tm of the amplicon.