Table 2.
Varroa mite specimens tested using the Varroa LAMP assay and 18S LAMP assay.
| Species | Origin | Varroa LAMP | 18S LAMP | Specimen number | GenBank accession | |||
|---|---|---|---|---|---|---|---|---|
| DNA extraction method | Time (min) | Temp (°C) | Time (min) | Temp (°C) | ||||
| V. destructor | CSIRO (New Zealand) | QE, ND | 18.25 | 77.7 | 24.25 | 88.3 | ||
| V. destructor | CSIRO (Texas-USA) | QE, ND | 14.25 | 77.9 | 20.25 | 88.6 | ||
| V. destructor | NZ PFR (New Zealand) | Chelex, D | 11.75 | 78.5 | 14.75 | 89.1 | VAITC9797b | OQ205274 |
| V. destructor | NZ PFR (New Zealand) | Qiagen, ND | 14.25 | 77.7 | 17.50 | 88.7 | ||
| V. destructor | NZ PFR (New Zealand) | Qiagen, ND | 13.25 | 77.9 | 17.25 | 88.6 | VAITC9768 | OQ205275 |
| V. destructor | NZ PFR (New Zealand) | Qiagen, D | 12.50 | 78.2 | 18.75 | 88.7 | VAITC9797c | OQ205276 |
| V. destructor | NZ PFR (New Zealand) | HotShot, ND | 10.75 | 78.2 | 16.50 | 88.8 | VAITC9797a | OQ205277 |
| V. destructor | CSIRO (unknown origin) | HotShot, ND | 11.75 | 78.4 | 18.75 | 89.0 | ||
| V. destructor | CSIRO (unknown origin) | HotShot, ND | 16.25 | 77.9 | 16.25 | 89.1 | ||
| V. destructor | CSIRO (Kuwait) | QE, ND | 23.50 | 77.7 | 15.75 | 88.6 | VAITC11190 | OQ205278 |
| V. destructor | CSIRO (unknown origin) | QE, ND | 16.25 | 78.7 | 13.25 | 88.7 | ||
| V. destructor | CSIRO (unknown origin) | QE, ND | 15.75 | 78.5 | 17.75 | 88.2 | ||
| V. destructor | CSIRO (unknown origin) | QE, ND | 16.00 | 78.3 | 13.25 | 88.9 | ||
| V. destructor | CSIRO (unknown origin) | QE, ND | 18.25 | 77.9 | 13.75 | 88.8 | VAITC11191 | OQ205279 |
| V. destructor | NZ PFR (New Zealand) | Xtract, ND | 20.00 | 78.1 | 19.75 | 88.3 | VAIC83854 | OQ205280 |
| V. destructor | NZ PFR (New Zealand) | Xtract, ND | 15.00 | 78.1 | 14.50 | 88.4 | VAIC84072 | OQ205281 |
| V. destructor | NZ PFR (New Zealand) | Xtract, ND | 14.00 | 78.4 | 20.00 | 88.1 | VAIC84073 | OQ205282 |
| V. jacobsoni | CSIRO (Papua New Guinea) | QE, ND | 21.50 | 77.3 | 23.75 | 88.5 | ||
| V. jacobsoni | NZ MPI (Fiji) | Qiagen, ND | 20.75 | 77.0 | 19.25 | 88.6 | ||
| V. jacobsoni | NZ MPI (Fiji) | Qiagen, ND | 17.00 | 77.3 | 24.25 | 84.8 | VAITC9771 | OQ205283 |
| V. jacobsoni | NZ MPI (Fiji) | Qiagen, ND | 18.50 | 77.3 | 21.75 | 84.7 | ||
| V. underwoodi | CSIRO (PNG) | QE, ND | 24.25 | 76.7 | 24.25 | 84.5 | VAITC11193 | OQ205284 |
| Tropilaelaps mercedesae | CSIRO (TC-177) | QE, ND | x | x | 37.75 | 84.2 | ||
| Melittiphis alvearius | AgVic (Victoria) | Qiagen, ND | x | x | 24.25 | 82.8 | ||
| Melittiphis alvearius | AgVic (Victoria) | Qiagen, ND | x | x | 24.00 | 83.9 | ||
| Melittiphis alvearius | AgVic (Victoria) | Chelex, ND | x | x | 18.00 | 85.2 | VAIC84153 | OQ205285 |
| Acarapis woodi | NZ MPI (Canada) | Chelex, ND | x | x | x * | x * | ||
| Acarapis externus | AgVic (Victoria) | Qiagen, ND | x | x | 36.50 | 88.5 | ||
| Acarapis externus | AgVic (Victoria) | Qiagen, ND | x | x | 39.25 | 88.5 | ||
| Machrochelidae | AgVic (Victoria) | QE, ND | x | x | 22.00 | 83.6 | VAITC9801a | OQ205286 |
| Machrochelidae | AgVic (Victoria) | QE, ND | x | x | 24.25 | 84.3 | VAITC9801b | OQ205287 |
*Sample DNA quality of this sample was confirmed through DNA barcoding.
x = No Amplification. Bold indicates Varroa species (target taxa). DNA extraction destructive (D), non-destructive (ND), QuickExtract (QE).