Table 3.
Comparison of different DNA extraction methods for V. destructor mites for use in Varroa LAMP assay: Destructive (laboratory-based) and Non-destructive (in-field compatible).
| DNA extraction methods | n | Varroa LAMP | 18S LAMP | ||
|---|---|---|---|---|---|
| Time (min) | Temperature (°C) | Time (min) | Temperature (°C) | ||
| Mean ± SD | Mean ± SD | ||||
| A. Destructive methods | |||||
| Chelex | 1 | 11.8 | 78.5 | 14.8 | 89.1 |
| Qiagen | 1 | 13.0 | 78.2 | 18.8 | 88.7 |
| B. Non-destructive methods | |||||
| HotShot | 3 | 12.7 ± 3 | 78.2 ± 0.3 | 17.0 ± 1.3 | 89.0 ± 0.1 |
| Qiagen | 2 | 13.6 ± 0.7 | 77.8 ± 0.1 | 17.3 ± 0.1 | 88.7 |
| QuickExtract | 7 | 17.4 ± 3 | 78.1 ± 0.4 | 16.7 ± 4.2 | 88.6 ± 0.2 |
| Xtract | 3 | 16.3 ± 3 | 78.2 ± 0.2 | 18.0 ± 3.0 | 88.2 ± 0.2 |