Table 4.
Testing of Varroa LAMP assay using multiple commercially available isothermal master mixes.
| Species | Varroa LAMP (35 min) | |||||
|---|---|---|---|---|---|---|
| GWS-K-VDES-08 | Isothermal mastermix ISO-001 | Isothermal mastermix ISO-004 | ||||
| Time (min) | Temp (°C) | Time (min) | Temp (°C) | Time (min) | Temp (°C) | |
| V. destructor | 11.3 | 78.6 | 12.2 | 77.4 | 11.0 | 77.9 |
| V. jacobsoni | 13.3 | 78.1 | 14.2 | 77.3 | 12.5 | 77.9 |
| V. underwoodi | 24.2 | 77.8 | 33.0 | 77.1 | 32.2 | 77.6 |
| Melittiphis alvearius | N/A | 0.0 | N/A | 0.0 | N/A | 0.0 |
| Varroa gBlock 1 × 106 | 19.0 | 80.6 | 14.2 | 79.9 | 13.3 | 80.3 |
| VDES-02 gBlock 3.8 × 108 | 9.2 | 80.8 | 8.5 | 80.1 | 7.2 | 80.4 |
| Neg | N/A | 0.0 | N/A | 0.0 | N/A | 0.0 |