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. 2023 Jul 24;14(7):461. doi: 10.1038/s41419-023-05926-6

Fig. 2. Hedgehog-GLI1 signal mediated the RNAP III signal pathway and tRNA synthesis to regulate cell cycle and death receptor binding in chondrosarcoma.

Fig. 2

A The expression of GLI1 was dichotomized using the median as a cutoff to define ‘high’’ or ‘low’’ expression categories for each sample obtained from GSE12475 (**P < 0.01). B Enrichment of BioPlanet signal pathways in GLI1high group. C mRNA expression changes of POU2F1, SMAPC1, POLR1B were detected using qPCR in GLI1high and GLI1low groups (*P < 0.05). D The GSEA enrichment plot showed RNA polymerase III transcription pathway is enriched in the GLI1high group (P < 0.01, NES = 2.48). E The heatmap representation of this subset of genes. F Volcano plot of significantly different endogenous tRNA fragments (tRFs) (|log2FC| > 1.0, *P < 0.05, **P < 0.01) between normal cartilage and chondrosarcoma, then we used the Genomic tRNA Database (GtRNAdb) to predict the tRNA gene. G Relative expression of tRNA-Asp-GTC, tRNA-Gly-TCC, tRNA-Gly-GCC, tRNA-Gly-CCC, tRNA-Lys-CTT, tRNA-Ser-GCT (*P < 0.05, **P < 0.01) between normal cartilage and chondrosarcoma. H, I Enrichment of GO Biological_Processes and Molecular Functions in tsRFuntion dataset (tsRFun).