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. 2023 Jul 24;14:4452. doi: 10.1038/s41467-023-40142-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — NMJs of MN4-Ib in third instar (a) non-ablated control (Is > GFP), (b) Is ablated (Is > GFP,hid,rpr), (c) non-ablated draper mutant (drpr^Δ5, Is > GFP) and (d) Is ablated draper mutant (drpr^Δ5, Is > GFP,hid,rpr) larvae, labeled with HRP (gray). The NMJ was expanded in control Is ablated larvae due to cross-neuron plasticity (b), and this expansion is absent in a draper mutant background (d). e Quantification of MN4-Ib bouton numbers in non-ablated and Is ablated larvae in control and drpr^Δ5 backgrounds. Control (N = 66 and 69 NMJs), t(133) = 5.030, p < 0.0001, unpaired t test, two-tailed. drpr^Δ5 (N = 53 and 56 NMJs), t(107) = 1.838, p = 0.0688, unpaired t test, two-tailed. f Cartoon representation of a dissected larva (pink) and a hemisegment highlighted by dashed red rectangle. The target muscle examined in this figure is shown in blue. Cartoon is generated with Biorender. g EPSP and mEPSP traces from non-ablated and Is ablated larvae in control and drpr^Δ5 backgrounds. h Quantification of EPSP amplitude of non-ablated and Is ablated larvae in control and drpr^Δ5 backgrounds. Control, t(41) = 4.924, p < 0.0001, unpaired t test, two-tailed. drpr^Δ5, t(48.04) = 7.011, p < 0.0001, unpaired t test, two-tailed, with Welch’s correction. Is ablated control vs Is ablated in drpr^Δ5, t(43.54) = 4.075, p = 0.0002, unpaired t test, two-tailed, with Welch’s correction. i Quantification of quantal content of non-ablated and Is ablated larvae in control and drpr^Δ5 backgrounds. Control, t(41) = 2.224, p = 0.0317, unpaired t test, two-tailed. drpr^Δ5, t(58) = 6.194, p < 0.0001, unpaired t test, two-tailed. Is ablated control vs Is ablated in drpr^Δ5, t(46) = 5.304, p < 0.0001, unpaired t test, two-tailed. For (h) and (i), N (NMJs) = 24, 19, 31, 29. j Quantification of normalized EPSP of Is ablated larvae in control and drpr^Δ5 backgrounds. Is ablated control vs drpr^Δ5, t(43.20) = 3.753, p = 0.0005, unpaired t test, two-tailed, with Welch’s correction. Is ablated control vs Ib/Ib+Is, t(29) = 6.506, p < 0.0001, unpaired t test, two-tailed. Is ablated in drpr^Δ5 vs Ib/Ib+Is, t(37.82) = 1.524, p = 0.1357, unpaired t test, two-tailed, with Welch’s correction. k Quantification of normalized quantal content of Is ablated larvae in control and drpr^Δ5 backgrounds. t(46) = 3.730, p = 0.0005, unpaired t test, two-tailed. For (j, k), N (NMJs) = 19 and 29. Error bars indicate ± SEM, ns = non-significant, *p < 0.05, ***p < 0.001, ****p < 0.0001.