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. 2023 Jul 24;14:4452. doi: 10.1038/s41467-023-40142-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–h NMJs of MN4-Ib in third instar non-ablated and Is ablated larvae in control, glia draper knockdown, muscle draper knockdown, and double knockdown backgrounds, labeled with GFP (green) and HRP (magenta). NMJ expansion was observed upon Is MN ablation (b), and this expansion is absent in double draper knockdown (d) and glial draper knockdown (h) backgrounds. i Cartoon representation of a dissected larva (pink) and a hemisegment highlighted by dashed red rectangle. The target muscle examined in this figure is shown in blue. Cartoon is generated with Biorender. j Quantification of MN4-Ib bouton number between non-ablated and Is ablated larvae in control, glia draper knockdown, muscle draper knockdown, and double knockdown backgrounds. Control (N = 20 and 21 NMJs), t(39) = 2.822, p = 0.0075, unpaired t test, two-tailed. Glia draper knockdown (N = 20 and 19 NMJs), t(37) = 0.7525, p = 0.4565, unpaired t test, two-tailed. Muscle draper knockdown (N = 16 and 15 NMJs), t(29) = 2.204, p = 0.0356, unpaired t test, two-tailed. Double knockdown (N = 21 and 16 NMJs), t(35) = 0.2965, p = 0.7686, unpaired t test, two-tailed. k Quantification of normalized EPSP of Is ablated larvae in control, glia draper knockdown, muscle draper knockdown, and double knockdown backgrounds. F(3, 85) = 9.191, p < 0.0001, One-way ANOVA. Is ablated control vs glia draper knockdown, p = 0.0001. Is ablated control vs muscle draper knockdown, p = 0.0042. Is ablated control vs double knockdown, p = 0.0006. Is ablated control vs Ib/Ib+Is, t(37) = 5.462, p < 0.0001, unpaired t test, two-tailed. Is ablated in glia draper knockdown vs Ib/Ib+Is, t(30) = 1.483, p = 0.1486, unpaired t test, two-tailed. Is ablated in muscle draper knock down vs Ib/Ib+Is, t(38) = 3.178, p = 0.0029, unpaired t test, two-tailed. Is ablated in double knockdown vs Ib/Ib+Is, t(24) = 1.540, p = 0.1367, unpaired t test, two-tailed. l Quantification of normalized quantal content of Is ablated larvae in control, glia draper knockdown, muscle draper knockdown, and double knockdown backgrounds. F(3, 85) = 8.263, p < 0.0001, One-way ANOVA. Is ablated control vs glia draper knockdown, p = 0.0028. Is ablated control vs muscle draperknock down, p = 0.9913. Is ablated control vs double knockdown, p = 0.0052. For (k, l), (NMJs) = 27, 20, 28, 14. Error bars indicate ± SEM, ns = non-significant, *p < 0.05, **p < 0.01, ***p < 0.001.