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. 2023 Jul 24;14:4452. doi: 10.1038/s41467-023-40142-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Cartoon representation of a dissected larva (pink) and a hemisegment highlighted by dashed red rectangle. The target muscle examined in (b–d) is shown in blue. Cartoon is generated with Biorender. b Quantification of MN4-Ib bouton number in non-ablated and Is ablated larvae in control, glia draper-II overexpression, and muscle draper-II overexpression backgrounds. Control (N = 19 and 24 NMJs), t(41) = 4.458, p < 0.0001, unpaired t test, two-tailed. Glia draper-II overexpression (N = 19 and 23 NMJs), t(40) = 1.440, p = 0.1578, unpaired t test, two-tailed. Muscle draper-II overexpression (N = 18 and 23 NMJs), t(39) = 1.114, p = 0.2720, unpaired t test, two-tailed. c Quantification of normalized EPSP of MN4-Ib in Is ablated larvae in control, glia draper-II overexpression, and muscle draper-II overexpression backgrounds. F(2, 35) = 1.535, p = 0.2296, One-way ANOVA. Is ablated control vs glia draper-II overexpression, p = 0.0466. Is ablated control vs muscle draper-II overexpression, p = 0.9980. Is ablated control vs Ib/Ib+Is, t(24) = 3.443, p = 0.0021, unpaired t test, two-tailed. Is ablated in glia draper-II overexpression vs Ib/Ib+Is, t(22) = 0.4045, p = 0.6897, unpaired t test, two-tailed. Is ablated in muscle draper-II overexpression vs Ib/Ib+Is, t(22) = 2.610, p = 0.0160, unpaired t test, two-tailed. d Quantification of normalized quantal content of MN4-Ib in Is ablated larvae in control, glia draper-II overexpression, and muscle draper-II overexpression backgrounds. F(2, 35) = 0.3700, p = 0.6934, One-way ANOVA. Is ablated control vs glia draper-II overexpression, p = 0.0370. Is ablated control vs muscle draper-II overexpression, p = 0.8123. For (c, d), N (NMJs) = 14, 12, 12. e Cartoon representation of a dissected larva (pink) and a hemisegment highlighted by dashed red rectangle. The target muscle examined in (f–h) is shown in blue. Cartoon is generated with Biorender. f Quantification of MN6-Ib bouton number in non-ablated and Is ablated larvae in control, glia draper-II overexpression, and muscle draper-II overexpression backgrounds. Control (N = 19 and 19 NMJs), t(36) = 4.319, p = 0.0001, unpaired t test, two-tailed. Glia draper-II overexpression (N = 23 and 17 NMJs), t(38) = 1.626, p = 0.1122, unpaired t test, two-tailed. Muscle draper-II overexpression (N = 16 and 20 NMJs), t(34) = 0.1763, p = 0.8611, unpaired t test, two-tailed. g Quantification of normalized EPSP of MN6-Ib in Is ablated larvae in control, glia draper-II overexpression, and muscle draper-II overexpression backgrounds. F(2, 34) = 2.731, p = 0.0795, One-way ANOVA. Is ablated control vs glia draper-II overexpression, p = 0.0680. Is ablated control vs muscle draper-II overexpression, p = 0.7116. Is ablated control vs Ib/Ib+Is, t(21) = 2.606, p = 0.0165, unpaired t test, two-tailed. Is ablated in glia draper-II overexpression vs Ib/Ib+Is, t(21) = 1.008, p = 0.3250, unpaired t test, two-tailed. Is ablated in muscle draper-II overexpression vs Ib/Ib+Is, t(19) = 2.162, p = 0.0436, unpaired t test, two-tailed. h Quantification of normalized quantal content of MN6-Ib in Is ablated larvae in control, glia draper-II overexpression, and muscle draper-II overexpression backgrounds. F(2, 34) = 8.539, p = 0.0010, One-way ANOVA. Is ablated control vs glia draper-II overexpression, p = 0.0130. Is ablated control vs muscle draper-II overexpression, p = 0.5614. For (g, h), N (NMJs) = 13, 13, 11. Error bars indicate ± SEM, ns = non-significant, *p < 0.05, **p < 0.01.