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. 2023 Jul 24;14:4452. doi: 10.1038/s41467-023-40142-y

Fig. 7. MN11-Ib displayed cross-neuron plasticity upon Is ablation.

Fig. 7

a, b NMJs of MN11-Ib in third instar control (Is > GFP) and Is ablated (Is > GFP,hid,rpr) larvae labeled with GFP (green) and HRP (magenta). Note the larger NMJs in Is ablated larvae. c Quantification of MN11-Ib bouton number in non-ablated (N = 31 NMJs) and Is ablated (N = 32 NMJs) larvae. t(61) = 3.408, p = 0.0012, unpaired t test, two-tailed. d Cartoon representation of a dissected larva (pink) and a hemisegment highlighted by dashed red rectangle. The target muscle examined in this figure is shown in blue (f–i). Cartoon is generated with Biorender. e EPSP and mEPSP recordings of muscle 11 from non-ablated and Is ablated larvae. f Quantification of mEPSP frequency. t(21) = 0.5408, p = 0.5943, unpaired t test, two-tailed. g Quantification of mEPSP amplitude. t(21) = 0.4446, p = 0.6611, unpaired t test, two-tailed. h Quantification of EPSP amplitude. t(18.02) = 3.840, p = 0.0012, unpaired t test, two-tailed, with Welch’s correction. i Quantification of quantal content. t(21) = 3.657, p = 0.0015, unpaired t test, two-tailed. For (f–i), N (NMJs) = 10, 13. Error bars indicate ± SEM, ns = non-significant, **p < 0.01.