Table 2.
Comparison of similarity score among the wild-type, − 117 Aγ (G>A), − 117 Aγ (G>C), and -114 Aγ (C>T) sequences using the online TFBIND program for prediction of CCAAT related transcription factors binding affinity.
| AC ID from TRANSFAC R.3.4 | Name of the binding factor | Consensus sequence | Wild type | − 117 Aγ (G>A)31,32 | − 117 Aγ (G>C) (this study) | − 114 Gγ (C>T)15 |
|---|---|---|---|---|---|---|
| M00254 V$CAAT_01 | CCAAT box (cellular and viral CCAAT box) | NNNRRCCAATSA | 0.91076 | 0.922056 | 0.824343 | 0.797797 |
| M00104 V$CDPCR1_01 | CDP CR1 (cut-like homeodomain protein) | NATCGATCGS | 0.80267 | 0.806578 | 0.798437 | < 0.77 |
| M00185 V$NFY_Q6 | NF-Y (nuclear factor Y (Y-box binding factor)) | TRRCCAATSRN | 0.939877 | 0.955036 | 0.922919 | 0.821172 |
| M00159 V$CEBP_01 | C/EBP (CCAAT/enhancer binding protein) | NNTKTGGWNANNN | < 0.87 | 0.888817 | < 0.87 | < 0.87 |
| M00190 V$CEBP_Q2 | C/EBP (CCAAT/enhancer binding factor) | NNNTTGCNNAANNN | 0.843209 | 0.869341 | 0.821862 | < 0.82 |
| M00116 V$CEBPA_01 | C/EBPalpha(CCAAT/enhancer binding protein alpha) | NNATTRCNNAANNN | 0.851779 | 0.851779 | < 0.80 | 0.829879 |
| M00109 V$CEBPB_01 | C/EBPbeta (CCAAT/enhancer binding protein beta) | RNRTKNNGMAAKNN | < 0.81 | 0.829371 | < 0.81 | < 0.81 |
| M00201 V$CEBP_C | C/EBP (C/EBP binding site) | NGWNTKNKGYAAKNSAYA | < 0.80 | 0.801226 | < 0.80 | < 0.80 |
| M00072 V$CP2_01 | CP2 | GCNMNAMCMAG | < 0.78 | 0.801887 | < 0.78 | < 0.78 |
S = C or G, W = A or T, R = A or G, Y = C or T, K = G or T, M = A or C, N = any base pair.