Skip to main content
. 2023 Jul 24;14:4448. doi: 10.1038/s41467-023-40242-9

Fig. 3. Production of highest de novo ferulic acid titer via modular strain and media optimization.

Fig. 3

A Modular scheme for bioproduction of ferulic acid from a glycerol feed, utilizing three of the heterologous enzymes detailed into Fig. 1. B p-coumaric acid titer achieved by Module I in modified MOPS media after 24 and 48 h culture in 2 ml tubes. C Titers of p-coumaric acid, caffeic acid, and ferulic acid after 63 h in modified MOPS media by Modules I and II resulting from systematic addition or subtraction of additional media components. D Heat map of p-coumaric acid, caffeic acid, and ferulic acid titers achieved via optimized media formulation across a range of Module I: Module II ratios. E 63 h titers achieved by optimized module and media conditions at 2 ml tube scale, demonstrating highest de novo ferulic acid production. Data are mean ± SD.; n = 3 biological replicates. Source data are provided as a Source Data file.