Fig. 4.

MC3T3E-1 cells migration properties and cell division direction. a The cell migration direction was presented as the ratio of the Y-axis migration length to the X-axis migration length. The Y-axis is defined as the stretch direction. b The mean migration velocity within each 6 h checkpoint. Data are presented as the mean ± SEM. *P < 0.05, One-way ANOVA followed by Bonferroni’s multiple comparison test. The numbers of evaluated cells for a and b: control, n = 16; continuous, n = 23; cyclic, n = 21. Three random fields per sample were examined under a × 20 objective. Each experiment was repeated at least three times. c The cell division direction was defined as the angle between the cell division axis and the stretch direction. Graphs show the data as jittered dots. The numbers of evaluated cells: control, 0 h = 13, 12 h = 34, 24 h = 21; continuous, 0 h = 14, 12 h = 30, 24 h = 12; cyclic, 0 h = 34, 12 h = 49, 24 h = 25. The horizontal bars in the middle indicate the median values for each group: control, 0 h = 45.0, 12 h = 41.9, 24 h = 40.2; continuous, 0 h = 35.0, 12 h = 37.5, 24 h = 30.6; cyclic, 0 h = 73.2, 12 h = 74.4, 24 h = 76.2. Vertical bars indicate 95% confidence intervals for each median