Fig. 4.

Immunofluorescence staining of integrin αvβ6 in human biliary tissue sections. Integrin αvβ6 was expressed on biliary epithelial layers of controls (left panel) and patients with PSC (right panel). A specific anti-integrin αvβ6 monoclonal antibody (10D5) [24] was used. The staining was abolished by preincubation with recombinant integrin αvβ6 (middle panel) but not other integrins (data not shown). Similar data were obtained from all patients (n = 10) and controls (n = 5) examined, and representative images are shown. The white boxes on the lower left are magnified images of the dashed line boxes. Scale bars: 100 μm. PSC primary sclerosing cholangitis