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. Author manuscript; available in PMC: 2023 Oct 23.
Published in final edited form as: Cell Rep. 2023 Jan 25;42(2):112026. doi: 10.1016/j.celrep.2023.112026

Figure 2. Dopamine controls MBn neurotransmission.

Figure 2.

(A) The GRAB ACh sensor was expressed in MBns using R13F02-gal4. One second of odor (MCH) was delivered at 5 s. n = 11.

(A′) Mean time series projection of GRAB ACh fluorescence for baseline, across 2 s prior odor delivery; for odor response, across 2 s starting at odor delivery. Scale bar, 10 μm.

(A″) Traces show the average response (±SEM, shading) across all flies tested.

(A″′) Average odor responses were quantified for each compartment.

(B) The GRAB ACh sensor was expressed in MBns using R13F02-LexA, and TNT was expressed in PAM DANs using R58E02 gal4. One second of odor (MCH) was delivered at 5 s. n = 8.

(B′) Mean time series projection of GRAB ACh fluorescence for odor responses, across 2 s starting at odor delivery. Scale bar, 10 μm.

(B″) Traces show the average odor (MCH) response (±SEM) across all flies tested in γ3.

(B″′) Average odor (MCH) responses were quantified for each compartment.

(C) The GRAB ACh sensor was expressed in MBns using R13F02-LexA, and NachBac was expressed in PAM DANs using R58E02 gal4. n = 8.

(C′) Mean time series projection of GRAB ACh fluorescence for odor (MCH) responses, across 2 s starting at odor delivery. Scale bar, 10 μm.

(C″) Traces show the average response (±SEM) across all flies tested in γ5.

(C″′) Average odor responses were quantified for each compartment in γ2–5.

Box-and-whisker plots showthe range of individual data points, with the interquartile spread as the box and the median as the line bisecting each box. *p < 0.05.

(A) One-way ANOVA with Dunn′s test. (B and C) One-way ANOVA with Kruskal-Wallis test.