Figure 3. The dDA1 dopamine receptor is required for MBn neurotransmission.
(A) The GRAB ACh sensor and RNAi transgenes were expressed in MBns using R13F02-gal4. One second of odor (MCH) vapor was delivered at 5 s. n = 8.
(A′) Mean time series projection of GRAB Ach fluorescence for odor responses, across 2 s starting at odor delivery. Scale bar, 10 μm.
(A″) Traces show the average odor (MCH) response (±SEM) across all flies tested.
(A′″) Average odor (MCH) responses were quantified for each compartment in γ2–5.
Box-and-whisker plots show the range of individual data points, with the interquartile spread as the box and the median as the line bisecting each box. *p < 0.05. (A) One-way ANOVA with Kruskal-Wallis test.