KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
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| BAPTA-AM | Sigma/Millipore | Cat# A1076 |
| Forskolin | Sigma | Cat# F6886 |
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| Experimental models: Organisms/strains | ||
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| D.melanogaster: tdtomato, w[*]; P{y[+t7.7] w[+mC] = 10XUAS-IVS-myr::tdTomato}attP2 | BDSC | RRID:BDSC_32221 |
| D.melanogaster: R1SF02-gal4, w[1118]; P{y[+t7.7] w[+mC] = GMR13F02GAL4}attP2 | BDSC | RRID:BDSC_48571 |
| D.melanogaster: TH-gal4, w[*]; P{w[+mC] = ple-GAL4.F3 | BDSC | RRID:BDSC_8848 |
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D.melanogaster: R58E02-gal4, w[1118]; P{y[+t7.7] w[+mC] = GMR58E02-GAL4}attP2 |
BDSC | RRID:BDSC_41347 |
| D.melanogaster: TNT, w[*]; P{w[+mC] = UAS-TeTxLC.tnt}Γ2 | BDSC | RRID:BDSC_28838 |
| D.melanogaster: NaChBac, y[1] w[*]; P{w[+mC] = UAS-NaChBac}2 | BDSC | RRID:BDSC_9469 |
| D.melanogaster: DAMB RNAi, y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMC′2893}attP2 | BDSC | RRID:BDSC_51423 |
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D.melanogaster: DOP2R RNAi, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02025} attP2y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02025}attP2 |
BDSC | RRID:BDSC_26001 |
| D.melanogaster: dDA1 RNAi-1, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HM04077}attP2 | BDSC | RRID:BDSC_31765 |
| D.melanogaster: dDA1 RNAi-2, P{KK102341}VIE-260B | VDRC | FBst0478881 |
| D.melanogaster: DUMB2, PBac{WH}Dop1R1f02676 | VDRC | FBal0184074 |
| D.melanogaster: Syt-GCaMP, w[*]; P{y[+t7.7] w[+mC] = UAS-sytGCaMP6s} attP40; | BDSC | RRID:BDSC_64415 |
| D.melanogaster: Control (S6S0S), y[1] v[1]; P{y[+t7.7] = CaryP}attP2 | BDSC | RRID:BDSC_36303 |
| D.melanogaster: Dunce RNAi, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02561} attP2 | BDSC | RRID:BDSC_27250 |
| D.melanogaster: Rutabaga RNAi-1, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02361} attP2 | BDSC | RRID:BDSC_27035 |
| D.melanogaster: Rutabaga RNAi-2, P{KK109441}VIE-260B | VDRC | FBst0473632 |
| D.melanogaster: Control (60100), y,w[1118]; P{attP,y[+],w[3′] | VDRC | 60100 |
| D.melanogaster: Control (60000), w[1118] | VDRC | 60000 |
| D.melanogaster: Control (S6S04), y[1] v[1]; P{y[+t7.7] = CaryP}attP40 | BDSC | RRID:BDSC_36304 |
| D.melanogaster. GCaMP6m-FusionRed (Gusion), | Greg Macleod | N/A |
| D.melanogaster. GRAB-DA (UAS), w[*]; P{y[+t7.7] w[+mC] = 10XUAS-GRAB(DA2m)} attP40 | BDSC | RRID.BDSC_90878 |
| D.melanogaster. GRAB-DA (lexA), w[*]; P{y[+t7.7] w[+mC] = 10XlexAop-GRAB(DA2m)} attP40 | BDSC | RRID.BDSC_90879 |
| D.melanogaster. GRAB-Ach (UAS, 2ND Chromosome), w[*]; P{y[+t7.7] w[+mC] = 10XUAS-GRAB(ACh3.0)}attP40 | BDSC | RRID.BDSC_86549 |
| D.melanogaster. GRAB-Ach (UAS, 3rd Chromosome), w[*]; PBac{y[+mDint2] w[+mC] = 10XUAS-GRAB (ACh3.0)}VK00005 | BDSC | RRID.BDSC_86550 |
| D.melanogaster. GRAB-Ach (lexAop, 2ND Chromosome), w[*]; P{y[+t7.7] w[+mC] = 10XlexAop-GRAB (ACh3.0)}attP40 | BDSC | RRID.BDSC_86551 |
| D.melanogaster. GRAB-Ach (lexAop, 3rd Chromosome), w[*]; PBac{y[+mDint2] w[+mC] = 10XlexAop-GRAB (ACh3.0)}VK00005 | BDSC | RRID.BDSC_86552 |
| D.melanogaster. Ac78C, y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMC′3999}attP2 | BDSC | RRID.BDSC_55312 |
| D.melanogaster. ACXD, y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.GL00425}attP2 | BDSC | RRID.BDSC_35589 |
| D.melanogaster. Pka-C2, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF01756}attP2 | BDSC | RRID.BDSC_31243 |
| D.melanogaster. CrebB, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02494} attP2 | BDSC | RRID.BDSC_29332 |
| D.melanogaster. Cngl, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF03099} attP2 | BDSC | RRID.BDSC_28684 |
| D.melanogaster. Pka-C2, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF01448} attP2 | BDSC | RRID.BDSC_31656 |
| D.melanogaster. CrebA, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02823}attP2 | BDSC | RRID.BDSC_27648 |
| D.melanogaster. Pka-C3, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02723}attP2 | BDSC | RRID.BDSC_27569 |
| D.melanogaster. Epac, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02476}attP2 | BDSC | RRID.BDSC_29317 |
| D.melanogaster. Pka-C1 RNAi-1, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF01218}attP2 | BDSC | RRID.BDSC_31277 |
| D.melanogaster. Pka-R1, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02788}attP2 | BDSC | RRID.BDSC_27708 |
| D.melanogaster. CngA, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02039}attP2 | BDSC | RRID.BDSC_26014 |
| D.melanogaster. CngB, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02034}attP2 | BDSC | RRID.BDSC_26009 |
| D.melanogaster: Pka-C1 RNAi-2, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF01188}attP2 | BDSC | RRID:BDSC_31599 |
| D.melanogaster. Pka-R2 RNAi-1, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02759}attP2 | BDSC | RRID:BDSC_27680 |
| D.melanogaster: CrebA, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02189}attP2 | BDSC | RRID:BDSC_31900 |
| D.melanogaster: AC3, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF03041}attP2 | BDSC | RRID:BDSC_28626 |
| D.melanogaster: Akap200, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HM05018}attP2 | BDSC | RRID:BDSC_28532 |
| D.melanogaster: CngB, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMJ21964}attP40 | BDSC | RRID:BDSC_58072 |
| D.melanogaster: ACXC, y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMC′6401} attP40 | BDSC | RRID:BDSC_67298 |
| D.melanogaster: ACXB, y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMC′5250}attP40 | BDSC | RRID:BDSC_62243 |
| D.melanogaster: CΓ43373, y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMC′5757}attP4 | BDSC | RRID:BDSC_64884 |
| D.melanogaster: Ac13E, y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMC′5254}attP40 | BDSC | RRID:BDSC_62247 |
| D.melanogaster: ACXD, y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMC′5344} attP40 | BDSC | RRID:BDSC_62871 |
| D.melanogaster: CΓ32301, y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMC′5252}attP40 | BDSC | RRID:BDSC_62245 |
| D.melanogaster: CΓ32305, y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMC′5253}attP40 | BDSC | RRID:BDSC_62246 |
| D.melanogaster: ACXA, y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMC′5251}attP40 | BDSC | RRID:BDSC_62244 |
| D.melanogaster: CrebB, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMJ30249} attP40/Cy0 | BDSC | RRID:BDSC_63681 |
| D.melanogaster: Pka-R2 RNAi-1, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMJ21276} attP40 | BDSC | RRID:BDSC_53930 |
| D.melanogaster: Ac3, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMJ30182} attP40 | BDSC | RRID:BDSC_63615 |
| D.melanogaster: CrebA, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMJ02218} attP40 | BDSC | RRID:BDSC_42562 |
| D.melanogaster: CΓ32301, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMJ21904} attP40 | BDSC | RRID:BDSC_57857 |
| D.melanogaster: CΓ43373, P{KK110535}VIE-260B | VDRC VDRC | FBst0473929 |
| D.melanogaster: Acxc, FBst0476995 P{KK103168}VIE-260B | VDRC | FBst0476995 |
| D.melanogaster: ACXB/AC34A, P{KK106308}VIE-260B | VDRC | FBst0476106 |
| D.melanogaster: Ac76E, P{KK105800}VIE-260B | VDRC | FBst0478057 |
| D.melanogaster: ACXD, P{KK106797}VIE-260B | VDRC | FBst0479218 |
| D.melanogaster: CΓ32305, P{KK106912}VIE-260B | VDRC | FBst0472976 |
| D.melanogaster: CΓ32305, w[1118]; P{GD14845}v36592 | VDRC | FBst0461757 |
| D.melanogaster: ACXA, w[1118]; P{GD16283}v49100 | VDRC | FBst0468273 |
| D.melanogaster: CΓ43373, w[1118]; P{GD13449}v23385 | VDRC | FBst0454980 |
| D.melanogaster: Acxc, w[1118]; P{GD1108}v2870 | VDRC | FBst0457608 |
| D.melanogaster: CΓ32301, w[1118]; P{GD3573}v12238 | VDRC | FBst0450451 |
| D.melanogaster: Ac13E, w[1118]; P{GD3374}v11547 | VDRC | FBst0450290 |
| D.melanogaster: CΓ32301, w[1118]; P{GD16147}v47917 | VDRC | FBst0467617 |
| D.melanogaster: ACXB, w[1118]; P{GD1109}v9748 | VDRC | FBst0471592 |
| D.melanogaster: Ac7SC, w[1118]; P{GD2764}v51979 | VDRC | FBst0469649 |
| D.melanogaster: Ac3, w[1118]; P{GD1799}v33217 | VDRC | FBst0459989 |
| D.melanogaster: Ac76e, w[1118] P{GD2737}v51974 | VDRC | FBst0469645 |
| D.melanogaster: ACXD overexpression, w[*]; P{w[+mC] = UAS-ACXD.D}3 | BDSC | RRID:BDSC_68218 |
| D.melanogaster: PKA-R2 RNAi-2, P{KK109446}VIE-260B | VDRC | FBst0473636 |
| D.melanogaster: 1471-gal4, w[1118]; P{w[+mW.hs] = GawB}1471 | BDSC | RRID:BDSC_9465 |
| D.melanogaster: THD′-gal4,; P{TH-D′-GAL4}; | BDSC | RRID:BDSC_93704 |
| D.melanogaster: MB296B split gal4, w[1118]; P{y[+t7.7] w[+mC] = R15B′1-p65.AD} attP40; P{y[+t7.7] w[+mC] = R26F01-GAL4.DBD}attP2 | BDSC | RRID:BDSC_68308 |
| D.melanogaster: MB′99C split gal4, w[1118]; P{y[+t7.7] w[+mC] = R73F07-GAL4.DBD}attP2 PBac{y[+mDint2] w[+mC] = ple-p65.AD}VK00027 | BDSC | RRID:BDSC_68290 |
| D.melanogaster: R25D′1-lexa, w[1118]; P{y[+t7.7] w[+mC] = GMR25D′1-lexA}attP40 | BDSC | RRID:BDSC_53519 |
| D.melanogaster: C305a-gal4, w[*]; P{w[+mW.hs] = GawB}Cka[c305a] | BDSC | RRID:BDSC_30829 |
| D.melanogaster: MB′77C split gal4, w[1118]; P{y[+t7.7] w[+mC] = R19F09-GAL4.DBD}attP2 PBac{y[+mDint2] w[+mC] = R25D′1-p65.AD}VK00027 | BDSC | RRID:BDSC_68284 |
| D.melanogaster: nACHRa1 RNAi, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF03103}attP2 | BDSC | RRID:BDSC_28688 |
| D.melanogaster: nACHRa2 RNAi, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02643}attP2 | BDSC | RRID:BDSC_27493 |
| D.melanogaster: nACHRa3 RNAi, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02750}attP2 | BDSC | RRID:BDSC_27671 |
| D.melanogaster: nACHRa4 RNAi, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF03419}attP2 | BDSC | RRID:BDSC_31985 |
| D.melanogaster: nACHRa5 RNAi, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF01963}attP2 | BDSC | RRID:BDSC_25943 |
| D.melanogaster: nACHRa6 RNAi, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF01853}attP2 | BDSC | RRID:BDSC_25835 |
| D.melanogaster: nACHRa7 RNAi, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.JF02570}attP2 | BDSC | RRID:BDSC_27251 |
| D.melanogaster: Dcr2, w1118; P{UAS-Dicer2}; | BDSC | RRID:BDSC_24650 |