KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-synapsin 1 (rabbit polyclonal) | Synaptic Systems | Catalog # 106 103; RRID:AB_11042000 |
| Anti-MAP2 (chicken polyclonal) | Synaptic Systems | Catalog # 188 006; RRID:AB_2619881 |
| Anti-synaptotagmin 7 (rabbit polyclonal) | Synaptic Systems | Cat# 105 173; RRID:AB_887838 |
| Anti-synaptotagmin 1 (rabbit polyclonal) | Synaptic Systems | Cat# 105 011; RRID:AB_887832 |
| Anti-GAPDH (rabbit monoclonal) | Cell signaling | Catalog # 14C10; RRID:AB_561053 |
| Anti-CSP-α (rabbit polyclonal) | Millipore | Catalog # AB1576; RRID:AB_90794 |
| Anti-syntaxin1 (mouse monoclonal) | Synaptic Systems | Catalog# 110 011; RRID:AB_887844 |
| Anti-SNAP25 (mouse monoclonal) | Synaptic Systems | Catalog #111 111; RRID:AB_887792 |
| Anti-synaptobrevin2 (mouse monoclonal) | Synaptic Systems | Catalog # 104 211; RRID:AB_887811 |
| Anti-BiP (rabbit polyclonal) | Abcam | Catalog # ab21685; RRID:AB_2119834 |
| Anti-CHOP (rabbit polyclonal) | Invitrogen | Catalog # PA5-86145; RRID:AB_2802940 |
| Anti-Ire1α (rabbit polyclonal) | Cell signaling | Catalog # 3294; RRID:AB_823545 |
| Anti-phospho-eIF2α (rabbit polyclonal) | Cell signaling | Catalog # 3597; RRID:AB_390740 |
| Anti-eIF2α (rabbit polyclonal) | Cell signaling | Catalog # 9722; RRID:AB_2230924 |
| Bacterial and virus strains | ||
| BL21-Gold(DE3) Competent E. coli | Agilent | Catalog #230132 |
| Chemicals, peptides, and recombinant proteins | ||
| mTESR Plus Medium | Stem Cell Technologies | Catalog# 100-0276 |
| DMEM-F12 Medium | Invitrogen | Catalog # 11330057 |
| N-2 Supplement | Invitrogen | Catalog # A1370701 |
| Insulin | Sigma | Catalog # I0516 |
| Puromycin | Stem Cell Technologies | Catalog # 73342 |
| DMEM Medium | Sigma | Catalog # D5796 |
| Fetal Bovine Serum | Fisher Scientific | Catalog # SH30070.03 |
| Penicillin-Streptomycin | Gibco | Catalog# 15140122 |
| 6-Cyano-7-nitroquinoxaline-2,3-dione disodium salt hydrate (CNQX) | Sigma-Aldrich | Catalog # C239 |
| D(−)-2-Amino-5-phosphonopentanoic acid (AP-5) | Sigma-Aldrich | Catalog # A8054 |
| Picrotoxin (PTX) | Sigma-Aldrich | Catalog # P1675 |
| Tetrodotoxin (TTX) | Enzo Life Sciences | Catalog # BML-NA120-0001 |
| Trypsin from bovine pancreas | Sigma-Aldrich | Catalog # T9935 |
| DNase I | Sigma-Aldrich | Catalog # D5025 |
| Matrigel | Corning | Catalog # 354234 |
| Neurobasal Plus Medium | GIBCO | Catalog # A3582901 |
| FuGENE 6 | Promega | Catalog # E2692 |
| QX-314 | EMD-Millipore | Catalog # 552233 |
| Transferrin | Calbiochem | Catalog # 616420 |
| Cytosine Arabinoside (Ara-C) | Sigma | Catalog # C6645 |
| B-27 supplement | GIBCO | Catalog # 17504-010 |
| Memantine | Sigma | Catalog # M9292 |
| Muscimol | Sigma | Catalog # M1523 |
| Kira6 | Selleck Chem | Catalog # S8658 |
| Salubrinal | Selleck Chem | Catalog # S2923 |
| Recombinant Human/Murine/Rat BDNF | PeproTech | Catalog # 45002 |
| Recombinant Human NT-3 | PeproTech | Catalog # 45003 |
| Mouse Laminin | Invitrogen | Catalog #23017015 |
| Growth-factor reduced matrigel | Corning | Catalog # 354230 |
| DMSO | Sigma | Catalog # D2650 |
| Experimental models: Cell lines | ||
| Human Embryonic Stem Cells (hESCs) | WiCell | Catalog #WA01 |
| Human embryonic kidney-293 (HEK293) cells | ATCC | Catalog # CRL-1573; RRID: CVCL_0045 |
| Experimental models: Organisms/strains | ||
| Sprague-Dawley rat pups (P2–P3) | Charles River | Strain code: 400 |
| CD1 mice pups (P2-P3) | Charles River | Strain code: 022 |
| Recombinant DNA | ||
| Plasmid: pRSV-REV (lentiviral packaging) | Dull etal., 199873 | Addgene# 12253 |
| Plasmid: pCMV-VSV-G (lentiviral packaging) | Stewart et al., 200374 | Addgene # 8454 |
| Plasmid: pMDLg/pRRE (lentiviral packaging) | Dull etal., 199873 | Addgene# 12251 |
| Plasmid: pFUW-TetO-hNgn2-EGFP-puroR | Ho et al, 201575 | Addgene #79823 |
| Plasmid:pFUW-rRTA | Hockemeyer et al, 200876 | Addgene #20342 |
| Plasmid:L307-Syt7-shRNA | Bacaj et al, 201329 | N/A |
| Plasmid:pFUW-Syt1 | Alten et al, 202277 | N/A |
| Plasmid:pFUW-CSPα | This paper | N/A |
| Software and algorithms | ||
| MiniAnalysis | Synaptosoft | http://www.synaptosoft.com/MiniAnalysis |
| Clampfit | Molecular Devices | https://www.moleculardevices.com |
| Axopatch | Molecular Devices | https://www.moleculardevices.com |
| Prism | GraphPad | https://www.graphpad.com:443/ |
| Intellicount | Fantuzzo et al., 201778 | N/A |