Table 2.
The effects of different sperm sorting techniques on sperm quality and pregnancy rates of IVF.
| Method | Rotation | Sperm quality | Pregnant rate | Reference | ||
|---|---|---|---|---|---|---|
| DGC | Horizontal rotor | Ratio of forward motile sperm (%) | 89.2 ± 8.5 | Clinical pregnancy rate (%) | 16.5 (30/182) | 22 |
| Sperm concentration (×106/ml) | 47.8 ± 14.3 | Abortion rate (%) | 3.3 (1/30) | |||
| Number of forward motile sperm (×106) | 13.6 ± 6.3 | Live birth rate (%) | 96.7 (29/30) | |||
| Ratio of normal sperm (%) | 18.5 ± 8.2 | |||||
| Angle rotor | Ratio of forward motile sperm (%) | 90.1 ± 8.8 | Clinical pregnancy rate (%) | 12.4 (21/170) | ||
| Sperm concentration (×106/ml) | 43.4 ± 15.1 | Abortion rate (%) | 9.5 (2/21) | |||
| Number of forward motile sperm (×106) | 12.9 ± 6.1 | Live birth rate (%) | 90.5 (19/21) | |||
| Ratio of normal sperm (%) | 14.2 ± 5.7 | |||||
| CSW | Sperm concentration (×106/ml) | 75.73 ± 52.09 | Clinical pregnancy rate (%) | 14.6 (56/328) | 26 | |
| Motility | 60.67 ± 16.65 | Live birth rate (%) | 10.9 (42/328) | |||
| Progressive motility | 49.25 ± 17.81 | Miscarriage (%) | 3.6 (14/328) | |||
| Inseminated total progressive motile sperm count (×106) | 40.18 ± 36.47 | |||||
| Density gradient CSW | Sperm concentration (×106/ml) | 63.67 ± 41.75 | Clinical pregnancy rate (%) | 12.5 (45/316) | ||
| Motility | 62.42 ± 15.89 | Live birth rate (%) | 10.8 (39/316) | |||
| Progressive motility | 49.25 ± 17.98 | Miscarriage (%) | 1.7 (6/316) | |||
| Inseminated total progressive motile sperm count (×106) | 24.82 ± 22.63 | |||||
| MACS | Sperm concentration (×106/ml) | 28.9 ± 26.1 (39) | Clinical pregnancy rate (%) | 65.8 (25/38) | 27 | |
| Sperm motility (%) | 17.8 ± 13.8 (39) | Implantation rate (%) | 53.6 (30/56) | |||
| Normal sperm morphology (%) | 2.7 ± 2.8 (39) | Early miscarriage rate (%) | 2.6 (1/38) | |||
| Control | Sperm concentration (×106/ml) | 35.8 ± 36.3 (41) | Clinical pregnancy rate (%) | 59.0 (23/39) | ||
| Sperm motility (%) | 23.2 ± 21.5 (41) | Implantation rate (%) | 55.6 (30/54) | |||
| Normal sperm morphology (%) | 2.6 ± 2.6 (41) | Early miscarriage rate (%) | 5.1 (2/39) | |||
| HA | Bound sperm (%) | 59.69 ± 7.24 | Clinical pregnancy rate (%) | 34 (32/93) | 28 | |
| Released sperm (%) | 31.16 ± 4.81 | Miscarriage rate (%) | 13 (4/32) | |||
| Progressive sperm (%) | 55.00 ± 5.00 | Live birth rate (%) | 17 (26/152) | |||
| Control | Bound sperm (%) | 45.23 ± 12.48 | Clinical pregnancy rate (%) | 7 (5/69) | ||
| Released sperm (%) | 17.72 ± 2.57 | Miscarriage rate (%) | 100 (5/5) | |||
| Progressive sperm (%) | 36.67 ± 3.33 | Live birth rate (%) | 0 (0/115) | |||
| PVP | Clinical pregnancy rate (%) | 21.6 (21/96) | ||||
| Abortion rate (%) | 14.3 (3/21) | |||||
| Live birth rate (%) | 94.7 (18/19) | |||||
CSW, conventional swim-up; DGC, density gradient centrifugation; HA, hyaluronic acid; IVF, in vitro fertilization; MACS, magnetic activated cell sorting; PVP, polyvinylpyrrolidone.