FIGURE 2.

Barcode frequency measurements to assess evolutionary dynamics in drug resistant cells. (A). Frequency measurements of each sample groups, namely, initial, DMSO and capecitabine resistant replicate A, B and C in Caco-2 cells are shown. (B). Frequency distributions of each sample groups are shown with the following samples: HT-29 cell line, initial, DMSO, irinotecan resistant replicate A, B and C. The phenotypes of barcodes are indicated with different colours (amber: pre-existing, olive green: de novo, silver grey: sensitive) on the right-hand side of bars. Barcodes with positive growth rate are classified as pre-existing or de novo (see “Methods” for the further details about barcode classification).