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. 2023 Jul 11;14:1178489. doi: 10.3389/fphar.2023.1178489

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Copyright © 2023 Danisik, Yilmaz and Acar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Temporal non-destructive analysis of clonal evolution in drug resistant cells. (A). Pre-existing and (B). de novo floating barcode frequencies of Caco-2 replicate B cell line under capecitabine treatment for duration of 6 months. (C). Pre-existing and (D). de novo floating barcode frequencies of HT-29 replicate B cell line under irinotecan treatment for duration of 6 months. Last data point demonstrating barcode frequency nearly matches the barcode frequency in harvested (resistant) sample point. Distinct colours represent each individual barcodes.