Figure 1.
Infection rates of BYDV-PAV and WDV (single inoculation (ISi )) on wheat plants of different ages at inoculation. Wheat plants of 7-, 9-,12-, 15- and 19-days old were inoculated with either BYDV-PAV (2 R. padi/plant, grey bars) or WDV (2 P. alienus/plant, white bars) for 24 hours IAP. Twenty-one days after inoculation, the sanitary status of each plant was tested by DAS-ELISA against BYDV-PAV and WDV for the calculation of the infection rates (ISi ). Experiments were carried out three times with series of 20 plants/virus species/DAI/replicate. Histogram bars and error bars represent mean and standard error of ISi , respectively. Letters (a and b) were obtained after statistical analysis of the data (GLM (family binomial) followed by EMM post-hoc test). For each virus species (in bold for WDV), different letters indicate a significant difference (α = 0.05) between plant age at the inoculation.