Skip to main content
. 2023 Jul 11;14:1194622. doi: 10.3389/fpls.2023.1194622

Figure 3.

Figure 3

Transmission rate (TSi ) (A) and viral load in BYDV-PAV and WDV source plants (B). Plants (7-days old at inoculation; 5 viruliferous R. padi or P. alienus for 24 h IAP) infected with BYDV-PAV (in grey) or WDV (in white), were used as source plants in transmission experiments. At 7 and 21 days of infection (DoI), virus-free insects were deposited on infected source plants for AAP (BYDV-PAV: 6 h; WDV: 24 h) before being transferred on 7-days old test plants (1 R. padi or 2 P. alienus per test plant). For each source plant, the sanitary status of test plants was individually tested (3 weeks after inoculation) for the calculation of TSi (A). In addition, source plants were sampled after each transmission experiment (at the end of AAP) and individually tested by (RT)-qPCR to evaluate BYDV-PAV and WDV viral loads (B). Histogram bars (A) and points (B) represent mean of Tsi and viral load, respectively. Error bars represent standard errors. These parameters were calculated from infected plants (BYDV-PAV; 7 DAI: n= 20, 21 DAI: n= 16; WDV; 7 DAI: n= 16, 21 DAI: n= 21) obtained after three repetitions of the experimental design. Letters (a and b) were obtained after statistical analysis of the data (GLM (family binomial) followed by EMM post-hoc test). For each virus species (in bold for WDV), different letters indicate a significant difference (α = 0.05) between DoI.