Figure 3.

NHE and NI can increase astrogliosis in the neurogenic niche. (A) Confocal microscopy images showing immunostaining for GFAP in the dentate gyrus. (B) Higher-magnification confocal microscopy images of dividing GFAP⁺ (Nestin-GFP-negative) cells in the GCL+SGZ after immunostaining for GFAP and BrdU. DAPI was used to stain cell nuclei in (A) and (B). (C) Proportion of dividing GFAP⁺ cells among the total population of GFAP⁺ cells. (D) Percentage of the area occupied by GFAP⁺ pixels in the molecular layer. Only GFAP⁺ cells that were Nestin-GFP-negative were counted. Nestin-GFP is omitted for better visualization. (E) Percentage of the area occupied by GFAP⁺ pixels in the hilus. The scale bar is 25 μm in (A) and 10 μm in (B). *p < 0.05, **p < 0.01, *p < 0.001 one-way ANOVA after all pairwise multiple comparisons by Holm-Sidak post hoc test. Bars show mean ± SEM. Dots show individual data.