Figure 4.

NHE and NI can cause cell death. (A) Confocal-microscopy images of the GCL stained for DAPI. DAPI DNA staining was used to identify apoptotic (pyknotic and karyorrhexic nuclei). (B) Quantification of the number of apoptotic nuclei. (C) Quantification of the GCL thickness, measured as the distance between the hilus and the molecular layer. **p < 0.01, ***p < 0.001 one-way ANOVA after all pairwise multiple comparisons by Holm-Sidak posthoc test Bars show mean ± SEM. Dots show individual data. The scale bar in (A) is 10 μm.