Figure 3.

Effects of Ca-EDTA, aztreonam and ceftazidime/avibactam and their combination on catheter biofilm infections

Effects of PBS (control), aztreonam (16 μg/mL), ceftazidime/avibactam (1 μg/mL) (16 μg/mL), ceftazidime-avibactam + aztreonem (16/16 μg/mL), Ca-EDTA (24 mg/mL), aztreonam -Ca-EDTA (4/12 mg/mL) and ceftazidime/avibactam- Ca-EDTA (4 μg/mL + 12 mg/mL) on carbapenemase-producing K. pneumoniae clinical isolates (n = 7) catheter biofilm infection model in vivo (A) biovolume (B) Live/Dead cell ratio (C) bio-volume inhibition, and (D) survival of mice. Values were measured each of the 10 catheters and represented the as averages. All experiments in A–E were performed as three biologically independent experiments, and the mean ± s.d. is shown. p values were determined using an unpaired, two-tailed Student’s t test.