Figure 4.

Confocal images of in vivo catheter biofilm

Confocal imaging analysis (3D) z axis image stack visualizing (40x magnification) of (A) aztreonam (16 μg/mL), (B) ceftazidime/avibactam (1 μg/mL) (16 μg/mL), (C) ceftazidime-avibactam + aztreonem (16/16 μg/mL), (D) Ca-EDTA (24 mg/mL), (E) aztreonam -Ca-EDTA (4/12 mg/mL) and (F) ceftazidime/avibactam- Ca-EDTA (4 μg/mL + 12 mg/mL) on carbapenemase-producing K. pneumoniae clinical isolates (n = 7) catheter biofilm infection model in vivo. One catheter was selected from the 10 catheters after confocal scanning in each treatment group for visualization purposes and live bacteria were stained green and dead bacteria were stained red.