REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
E. coli ATCC 25922	ATCC	25922
P. aeruginosa ATCC 27853	ATCC	27853
Chemicals, peptides, and recombinant proteins
Imipenem	Sigma Aldrich	10160
Meropenem	Sigma Aldrich	M2574
Ertapenem	Sigma Aldrich	SML1238
Doripenem	Sigma Aldrich	SML1220
Ceftazidime–avibactam	GlaxoSmithKline	AVYCAZ
Aztreonam	Thermo Fisher Scientific	J62887.03
Phosphate-buffered saline	Thermo Fisher Scientific	003002
Müller–Hinton agar	Sigma Aldrich	70191
Müller–Hinton II broth	Sigma Aldrich	90922
Ca-EDTA	Sigma Aldrich	304695-78-1
Porcine mucin	Sigma Aldrich	84082-64-4
Dexamethasone	Thermo Fisher Scientific	A17590.03
Ketamine	Sigma Aldrich	K-113
Isoflurane	Sigma Aldrich	792632
Fetal bovine serum	Gibco™; Waltham, MA	1962
Chlorhexidine	Thermo Fisher Scientific	214980050
Ampicillin sodium powder	Fisher BioReagents™	BP1760-5
Iodine isopropanol	Sigma-Aldrich	148938
Ethyl alcohol	Thermo Fisher Scientific	224095000
SYBR™ Green	Thermo Fisher Scientific	S7563
Critical commercial assays
PrestoBlue™ HS Cell Viability Reagent	Thermo Fisher Scientific	P50200
Live/Dead Bacterial Viability kit	Thermo Fisher Scientific	L7007
GeneJET RNA Purification Kit	Thermo Fisher Scientific	K0731
STET lysing solution	BD Bioscience	349202
qScript™ cDNA Synthesis Kit	QuantaBio	101414-100
Experimental models: Organisms/strains
C57BL/6 mice	Nomura Siam International (Bangkok, Thailand)	https://nomura-siam.com/en/
Oligonucleotides
16srRNA - for AGAGTTTGATCCTGGCTCAG 16srRNA - rev GGTTACCTTGTTACGACTT	Daikos et al.6	Daikos et al.6
16srRNA – for AGAGTTTGATCCTGGCTCAG 16srRNA – rev GGTTACCTTGTTACGACTT	Wannigama et al.29/Vuotto et al.30	Wannigama et al.29/Vuotto et al.30
kfu – for GGCCTTTGTCCAGAGCTACG kfu – rev GGGTCTGGCGCAGAGTATGC	Wannigama et al.29/Vuotto et al.30	Wannigama et al.29/Vuotto et al.30
ybtS – for GACGGAAACAGCACGGTAAA ybtS- rev GAGCATAATAAGGCGAAAGA	Wannigama et al.29/Vuotto et al.30	Wannigama et al.29/Vuotto et al.30
mrkD – for AAGCTATCGCTGTACTTCCGGCA mrkD – revGGCGTTGGCGCTCAGATAGG	Wannigama et al.29/Vuotto et al.30	Wannigama et al.29/Vuotto et al.30
luxS – for AGTGATGCCGGAACGCGG luxS – rev CGGTGTACCAATCAGGCTC	Wannigama et al.29/Vuotto et al.30	Wannigama et al.29/Vuotto et al.30
ompK35 – for GCAATATTCTGGCAGTGGTGATC ompK35 – rev ACCATTTTTCCATAGAAGTCCAGT	Wannigama et al.29/Vuotto et al.30	Wannigama et al.29/Vuotto et al.30
ompK36 – for TTAAAGTACTGTCCCTCCTGG ompK36 – rev TCAGAGAAGTAGTGCAGACCGTCA	Wannigama et al.29/Vuotto et al.30	Wannigama et al.29/Vuotto et al.30
uge – for TCTTCACGCCTTCCTTCACT uge – rev GATCATCCGGTCTCCCTGTA	Wannigama et al.29/Vuotto et al.30	Wannigama et al.29/Vuotto et al.30
wabG- for ACCATCGGCCATTTGATAGA wabG – rev CGGACTGGCAGATCCATATC	Wannigama et al.29/Vuotto. et al.30	Wannigama et al.29/Vuotto et al.30
Software and algorithms
ggplot2 3.3.5	R software package	https://www.r-project.org
PHLIP software		https://sourceforge.net/projects/phlip/
MATLAB		https://www.mathworks.com/products/matlab.html
Other
25-mm catheters	NIPRO	www.nipro.co.jp