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. 2023 Jun 30;26(7):107232. doi: 10.1016/j.isci.2023.107232

Figure 1.

Figure 1

TNFα-stimulated increase in E-selectin expression in un-transfected HUVECs

(A) Confocal images of TNFα-stimulated E-selectin expression in wild-type (WT) HUVECs and immortalized TERT2-HUVECs. Cells were stimulated with 1 nM TNFα for 6 (WT HUVECs) or 8 h (TERT2-HUVECs). H33342-stained nuclei are shown in blue and Alexa Fluor 488-labeled E-selectin in green. Scale bar represents 50 μm.

(B–D) Quantification of E-selectin expression in WT HUVECs using an alkaline phosphatase secondary antibody and p-nitrophenol phosphate substrate. Values show mean ± S.E.M. of the optical densities obtained in five separate experiments in response to 1 nM TNFα.

(C and D) Concentration-response curve (C) and time course (D) for TNFα-stimulated E-selectin expression in WT HUVECs. Values are mean ± S.E.M. of five separate experiments each performed in triplicate. In (C), values have been normalized to the response obtained with 1 nM TNFα. In (D), 1 nM TNFα was used, and values have been normalized to the response obtained at 8 h. ∗∗p < 0.001 compared to vehicle control (paired t test).