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. 2023 Jun 30;26(7):107232. doi: 10.1016/j.isci.2023.107232

Figure 3.

Figure 3

TNFα-stimulated nanoluciferase luminescence in HiBiT-E-selectin-edited HUVECs and HiBiT-edited TERT2-HUVECs

(A, C, and E) Increase in luminescence (relative light units) under basal conditions and in response to 6 h stimulation with 1 nM TNFα in HiBiT genome-edited HUVECs (A), mixed populations of HiBiT-edited TERT2-HUVECs (C) and HiBiT TERT2 clone C8 HUVECs (E).

(B, D, and F) Concentration-response curves for TNFα-stimulated luminescence in HiBiT HUVECs (B), HiBiT TERT2-HUVEC mixed populations (D), and HiBiT TERT2 clone C8 HUVECs (F). NanoBiT complementation was achieved by addition of purified LgBiT (50 nM) and furimazine substrate (1:400), and the plates were read using an BMG PHERAstar FS plate reader (450 nm, 30 nm bandpass). Values are mean ± S.E.M of 5 replicate experiments each performed in triplicate. ∗p < 0.05 compared to vehicle control (paired t test).