Figure 6.

Effect of histamine and VEGF₁₆₅a on HiBiT E-selectin expression in gene-edited TERT2-HUVEC clone C8

(A and B) Increase in luminescence under basal conditions and in response to 8 h stimulation with (A) 100 nM histamine or (B) a combination of 100 nM histamine and 1 nM TNFα in HiBiT genome-edited clone C8 TERT2-HUVECs.

(C and D) Increase in luminescence under basal conditions and in response to 8 h stimulation with 100 nM VEGF₁₆₅a (C) or (D) a combination of 100 nM VEGF₁₆₅a and 1 nM TNFα in HiBiT genome-edited clone C8 TERT2-HUVECs. Values represent mean ± S.E.M of the five independent experiments, each performed in triplicate. ∗p < 0.05 compared to vehicle control (paired t test). ∗∗p < 0.01 compared to vehicle control or #p < 0.05 compared to TNFα alone (One-way ANOVA).