Skip to main content
. 2023 Jun 30;26(7):107232. doi: 10.1016/j.isci.2023.107232

Table 1.

EC50 values for cytokine-stimulated HiBiT E-selectin expression in gene-edited wild-type and TERT2-HUVECs

WT-HUVECs EC50, nM (n) Clone C8 TERT2-HUVECs EC50 nM (n)
TNFα 0.06 ± 0.01 (5) 0.06 ± 0.02 (5)
LPS 10.00 ± 2.86 (5) 2.80 ± 0.70 (5)
IL-1α 41.04 ± 18.08 (5) 32.84 ± 5.73 (5)
IL-1β 48.26 ± 15.47 (5) 30.90 ± 6.23 (5)

Values represent mean ± S.E.M. of five independent experiments. In each experiment, triplicate determinations were made. WT-HUVECs are mixed populations of gene-edited (HiBiT-tagged E-selectin) HUVECs while the TERT2-HUVECs represent a clonal cell line homozygous for HiBiT-tagged E-selectin.