Table 2.
Quality control metrics to assess the impact of post-dissociation parameters
| 0.38mm Standard Tubing | 0.51 mm Tip Loading | 4:1 All Cell Chip Dilution | ≥1:1 All Cell Chip Dilution | |
|---|---|---|---|---|
| Contamination metrics | ||||
| Empty Droplet Scaled Slope Sum | 0.62 | 0.31 | 0.82 | 0.56 |
| Inverted Max Secant Distance | 0.68 | 0.46 | 0.83 | 0.56 |
| Inverted Secant Line St.Dev. | 0.40 | 0.34 | 0.45 | 0.36 |
| Inverted AUC Percentage | 0.58 | 0.34 | 0.76 | 0.42 |
| Avg. Percent Counts Ambient | 15.52 | 3.07 | 19.24 | 4.59 |
| Standard metrics | ||||
| Total Number of Cells | 1019.67 | 2880.75 | 156.00 | 376.67 |
| Avg. Percent Counts Mitochondria | 4.03 | 4.25 | 3.54 | 4.06 |
| Avg.Total Genes per Cell | 3682.52 | 2803.87 | 1857.88 | 2475.67 |
| Avg. Total Transcripts per Cell | 12509.90 | 7702.99 | 5408.91 | 8095.85 |
| AmbiQuant | ||||
| Overall score | 0.57 | 0.36 | 0.70 | 0.46 |