REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Blood cells	healthy volunteers	anonymous
Chemicals, peptides, and recombinant proteins
CO-101244	Tocris Bioscience	Cat # 2456
Phorbol 12-myristate 13-acetate	Tocris Bioscience	Cat # 1201
NMDA	Tocris Bioscience	Cat # 0114
(R)-CPP	Tocris Bioscience	Cat # 0247
Minimal Essential Media	Thermo Fisher	Cat # 21090-022
HBSS	Thermo Fisher	Cat # 14185-045
B27	Thermo Fisher	Cat # 12587010
HEPES solution	Merck	Cat #H0887-20ML
Minimal Essential Media	Thermo Fisher	Cat # 21090-022
Alexa Fluor 594 hydrazide	Thermo Fisher	Cat. #A10438
Fluo-4/AM	Thermo Fisher	Cat. #F-14201
Glycine	Tocris Bioscience	Cat. #0219
CPP	Sigma-Aldrich	Cas #:100828-16-8
MCPG	Tocris Bioscience	Cat. #0336
L-Glutamic dehydrogenase	Sigma-Aldrich	Cat. # 9029-12-3
β-Nicotinamide adenine dinucleotide hydrate	Sigma-Aldrich	N7004-1G
Cell tracker red	Thermo Fisher	C34552
Pluronic™ F-127	Thermo Fisher	Cat. #P-6866
Histopaque®-1077	Sigma-Aldrich	Cat. #1077
HEPES	Sigma-Aldrich	Cas #: 7365 45 9
HBSS (-Mg2+/−Ca2+)	Thermo Fisher	Cat. #14025092
Poly-D-Lysine	Thermo Fisher	Cat. # A3890401
Dextran	Sigma-Aldrich	Cas #: 9004-54-0
PBS	Sigma-Aldrich	Cat. #P4417
(+)-MK-801 hydrogen maleate	Sigma Aldrich	Cat. #M107
L-Glutamic acid monosodium salt monohydrate	Sigma-Aldrich	49621
D-APV	Abcam	Cat. #ab120003
DL-AP5	Bio-Techne Ltd	Cat. # 0105
NBQX disodium salt	Abcam	Cat. #ab120046
Pertussis toxin	Tocris Bioscience	Cat # 3097
Software and algorithms
pClamp 10	Molecular Devices	RRID:SCR_011323
ImageJ Fiji	NIH	RRID:SCR_003070
OriginPro	OriginLab Inc.	RRID:SCR_014212
Other
Digidata 1440A digitizer	Molecular Devices	RRID:SCR_021038
MultiClamp 700B amplifier	Molecular Devices	RRID:SCR_018455
Olympus Fluoview1000	Olympus	RRID:SCR_014215
Olympus BX51 Fluorescence Microscope	Olympus	RRID:SCR_018949
Micromanager4.1	NIH	https://micro-manager.org/
Evolve 512 EMCCD camera	Photometrics	https://www.photometrics.com/products/emccd-family