表 1.
质谱检索参数
Spectral search parameters
| Items | Settings |
| Static modification | TMT (N-tem, K); Carbamidomethyl (C) |
| Dynamic modification | Oxidation (M), Acetyl (N-term) |
| Digestion | Trypsin |
| Instrument | Orbitrap Fusion |
| MS1 tolerance | 10ppm |
| MS2 tolerance | 0.02Da |
| Missed Cleavages | 2 |
| Database | Nuiprot-reviewed_yes+taxonomy_9606.fasta |