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IFNGR1棕榈酰化检测及IFNGR1-WT和IFNGR1-C122A细胞构建
IFNGR1 palmitoylation assay and construction of IFNGR1- WT and IFNGR1-C122A cells. A: Effect of 2-BP on IFNGR1 protein expression in KYSE70 cells detected by immunoblotting showed IFNGR1 protein expression. B: PCR detection of IFNGR1-WT and IFNGR1-C122A plasmid constructs. C: Immunofluorescence detection of IFNGR1-WT and IFNGR1-C122A plasmid transfection efficiency (×100). D: Click-iT for detecting IFNGR1-WT and IFNGR1-C122Apalmitoylation levels.
