图 3.

LRG1通过增强TGF-β1信号调控脂肪变性肝细胞CM诱导的肝巨噬细胞活化

LRG1 regulates the activation of hepatic macrophages induced by conditioned medium (CM) derived from steatotic hepatocytes by enhancing TGF-β1 signaling. A: Primary hepatic macrophages were stimulated with CM derived from steatotic hepatocytes for 12 h, and were then treated with LRG1 (1 μg/mL), TGF-β1 (10 ng/mL) or both for 24 h. Cell morphological changes were observed under an inverted phase contrast microscope. B: Protein expression levels of iNOS detected by Western blotting. C: RT-qPCR for detecting mRNA expression levels of iNOS, CXCL-1 and IL-1β. Scale bar, 10 μm. Data are presented as Mean±SE (n=6). *P < 0.05.