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. 2023 Jul 24;6(10):e202302001. doi: 10.26508/lsa.202302001

Figure 4. Metal ion-dependent conformational changes of the A. aeolicus MutL (aqMutL) C-terminal endonuclease domain (CTD).

Figure 4.

(A) The catalytic site of the zinc-restored aqMutL CTD, in which cadmium ions in the crystal were replaced with zinc ions. Blue mesh represents the 2Fo–Fc map shown at 3.0 σ. Gray and red spheres represent zinc ions and water molecules, respectively. The guanidino group of Arg406 could not be identified. (B) Superimposition of the overall structures of the zinc-restored, zinc-bound (cocrystallized with zinc ions), and cadmium-bound (cocrystallized with cadmium ions) forms of the aqMutL CTD. (C) The catalytic site of the manganese-bound form of the aqMutL CTD. Blue mesh represents the 2Fo–Fc map shown at 3.0 σ. Purple spheres indicate manganese ions. The guanidino group of Arg406 could not be identified. (D) Superimposition of the overall structures of the manganese-bound, zinc-bound, and cadmium-bound forms of the aqMutL CTD.