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. 2023 Jul 24;6(10):e202302001. doi: 10.26508/lsa.202302001

Figure S2. Six manganese ions were found in the manganese-bound form of the A. aeolicus MutL C-terminal endonuclease domain.

Figure S2.

(A) The M4, M5, and M6 manganese ions were coordinated by Asp366, Glu384, and Glu416, respectively. (B) The endonuclease activity of the WT, D366A, E384A, and E416A mutant forms of the A. aeolicus MutL C-terminal endonuclease domain was measured in the manganese-containing buffer by using a supercoiled plasmid DNA as the substrate at 60°C for 10 min. The percentages of the incised DNA molecules were plotted against the protein concentrations. Average values from three independent experiments are shown with standard deviations.