Figure S4. Cadmium did not interfere with the DNA binding of A. aeolicus MutL (aqMutL).

(A) The DNA-binding ability of zinc- or cadmium-bound aqMutL was examined by electrophoretic mobility shift assay. After treatment with 0.5 mM ZnCl₂ or CdCl₂ at 60°C for 24 h, aqMutL was used for the assay. (A, B) Percentages of the shifted DNA signals in panel (A) were plotted against protein concentrations. Orange and cyan represent zinc- and cadmium-bound forms of aqMutL, respectively. Average values from three independent experiments are shown with standard deviations.

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