Figure 1. Full-length isoform sequencing and analysis workflow.

(A) Diagram of experimental setup and sequencing strategy. RNA was extracted from whole cell lysate and converted to cDNA using a poly(A) tail selection strategy. WT and mutant conditions were sequenced in triplicate. Each sequencing run was conducted in parallel, in which a WT or mutant was sequenced on separate row cells. (B) Data processing pipeline workflow. Full-Length Alternative Isoform analysis of RNA was used to construct a reference transcriptome from long-read data with matched short-read RNA-seq and to perform differential expression and productivity analyses.