Figure 3. Nanopore data recapitulates U2AF1 S34F splicing signature.

(A) Alternative splicing events that were found to be significantly altered between WT and U2AF1 S34F conditions. Events are broken down into different patterns of alternative splicing. (B) Change in percent spliced in correlation between short- and long-read cassette exon events. (C) Motifs of 3′ splice sites for altered cassette exons (left panels) and alternative acceptor sites (right panels) identified using nanopore data. (D) Alternative polyadenylation site selection schematic. (E) Ranked genes with significant changes in alternative polyadenylation site usage.