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. 2023 Jul 24;6(10):e202201886. doi: 10.26508/lsa.202201886

Figure S1. The gene expression of molecular clock and fibrosis-related genes is up-regulated in adenine and folic acid nephropathy (FAN)-induced nephrotoxicity.

Figure S1.

(A) Relative mRNA levels of Arntl and Tgfb 3, 7, 15, and 25 d after unilateral ureteral obstruction (UUO). (B, C) Heat map shows the relative mRNA expression of molecular clock genes, Tgfb and Fn1 in kidneys from mice subjected to (B) FAN for 7 and 15 d (n = 6 mice per condition) or (C) adenine for 25 d (n = 5 mice per condition). Red rectangles denote significant differences compared with vehicle-treated kidneys (Wilcoxon test). (B, C, D, E) Spearman r correlation of the data represented in (B, C), respectively. *P < 0.05. (F, G) Representative microphotographs of H&E and Sirius red stains from contralateral and obstructed kidneys of WT mice 3, 7, 15, and 25 d after UUO (F), 15 d after FAN and 25 d after AND (G). Scale bar: 150 μm. (F, H) Violin plots show the quantification of Sirius red in kidney sections of the analysis performed in (F), data are represented as the median with interquartile range. *P < 0.05 compared with control kidney. #P < 0.05, ##P < 0.01 compared with other time points, $$P < 0.01 compared with the UUO model (Mann–Whitney).