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. 2023 Jul 24;6(10):e202302101. doi: 10.26508/lsa.202302101

Figure 6. Reversible reduction of PRC1 binding to polycomb target loci following hexanediol treatment.

Figure 6.

(A) Heatmap representation (bottom panel) and average metaplots (top panel) of RING1B calibrated ChIP-seq signal distribution at merged RING1B peaks in untreated, 2,5-HD–treated, 1,6-HD–treated, and recovered WT mESCs. (B) IGV (Robinson et al, 2011) track views of RING1B cChIP-seq data from untreated, 2,5-HD–treated, 1,6-HD–treated, and recovered mESCs at the HoxD (top), Irx3, 5, 6 (middle), En2, Shh, and Mnx1 (bottom) genomic regions. (C) As for B but for the 50 Mb region of chromosome 2 tiled with oligonucleotide probes. Biological replicate for these data are in Fig S5. Genomic co-ordinates (Mb) are for the mm10 genome assembly. (D, E) qPCR analysis of ChIP for RING1B (D) and H3K27me3 (E) at Hoxd10, En2, and β-actin in untreated, 2,5-HD-treated, 1,6-HD–treated, and recovered mESCs. Enrichment is shown as mean percent input bound ± s.e.m. over two (β-actin) to four biological replicates.