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. 2023 Jul 24;6(10):e202302101. doi: 10.26508/lsa.202302101

Figure S5. Replicate data for Fig 6 showing reversible reduction of PRC1 binding to polycomb target loci following hexanediol treatment.

Figure S5.

(A) Heatmap representation (bottom panel) and summary metaplots (top panel) of RING1B calibrated ChIP-seq signal distribution at RefSeq gene TSS (±5 kb) enriched for marks in WT ESCs in untreated, 2,5-HD–treated, 1,6-HD–treated, and recovered mESCs. (B) IGV (Robinson et al, 2011) track views of Ring1B cChIP-seq data from untreated, 2,5-HD–treated, 1,6-HD–treated, and recovered mESCs at the HoxD (top), Irx3, 5, 6 (middle), En2, Shh, and Mnx1 (bottom) genomic regions. (C) As for B but for the 50 Mb region of chromosome 2 tiled with oligonucleotide probes. Genomic co-ordinates (Mb) are for the mm10 genome assembly.