Figure 3.
SDS-PAGE gel separation of human gzms A and K used in this study. The human gzms A and K were produced as inactive enzymes containing an N terminal His6-tag and an enterokinase site. The enzymes were both produced in the human cell line HEK293-EBNA with the episomal vector pCEP-Pu2. Entrokinase (EK) was used to cleave of the N-terminal tail to activate the two enzymes. The inactive enzymes with the N-terminal purification tag and the active enzymes were analyzed by separation on SDS-PAGE gel and visualized with Coomassie Brilliant Blue staining. PAGE Ruler was used as marker. M, marker; +EK, with entero-kinase; -EK, without enterokinase.