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. 2023 Jul 11;14:1211295. doi: 10.3389/fimmu.2023.1211295

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Copyright © 2023 Aybay, Ryu, Fu, Akula, Enriquez, Hallgren, Wernersson, Olsson and Hellman

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phage display sequences generated after cleavage by human gzms A and K after six rounds of selection. After the last round of selection, the released phages were collected for sequencing. The amino acid sequences are P-G-G-?-?-?-?-?-?-?-?-?-H-H-H-H-H-H and the “?” means randomized amino acids. The amino acid sequences were aligned into a P5-P4’ consensus. The cleavage occurs between positions P1 and P1’. The classification of amino acids was indicated by color based on the side chain properties located in the right bottom corner of the figure. In (A) we show the results for gzm A and in (B) for gzm K Web Logo presentations of the target selectivity of human gzms A and K are displayed in (C, D), respectively.