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. 2023 Jul 11;14:1211295. doi: 10.3389/fimmu.2023.1211295

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Copyright © 2023 Aybay, Ryu, Fu, Akula, Enriquez, Hallgren, Wernersson, Olsson and Hellman

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gating strategy and post-sort purity check for the isolation of human peripheral blood cell subpopulations. Blood samples collected from buffy coats were used to purify peripheral blood mononuclear cell populations. Cells were stained with antibodies against CD4, CD8, CD14 and CD19 to isolate CD8⁺ T cells, CD4⁺ T cells, CD19⁺ B cells and CD14⁺ monocytes. (A) Gating strategy to identify the different subpopulations. (B) A fraction of sorted cells were re-analyzed by flow cytometry after the sort to determine the purity of each isolated cell population. The figure is a representative sample of the two different samples used for the experiments.