Table 1.
Ampliseq analysis of the expression of five inflammatory cytokines and chemokines during in vitro culture for 4 hours of human monocytes in the presence or absence of 1 ug/ml of LPS (4h LPS) or 10 ug of recombinant active human gzm A (4h GzmA).
| 0 | 4h | 4h-LPS | 4h Gzm A | |
|---|---|---|---|---|
| IL-1α | 0 | 6 | 2745 | 56 |
| IL-1β | 12 | 72 | 43189 | 638 |
| TNF-α | 86 | 61 | 2955 | 271 |
| IL-6 | 0 | 1 | 8386 | 20 |
| IL-8 | 176 | 181 | 46829 | 2418 |
Controls included cell cultured for 4h in culture medium without any additions (4h) and cells analyzed before in vitro culturing (0), directly after purification according to previously published procedure (26).