Table 2.

Ampliseq analysis of four different peripheral blood immune cell populations.

Gene	B-cell	B-cell	Mono- cyte	Mono- cyte	CD4 T-cell	CD4 T-cell	CD8 T-cell	CD8 T-cell
Granule Proteases
GZMA	0.1	0	0.4	0.1	68	38	219	268
GZMK	0	0	0	0.1	148	51	228	146
GZMH	0	0	0	0	9	0.4	280	103
GZMB	0.4	0.1	0	0	2	0.6	155	70
GZMM	0	0	0	0.2	65	41	160	279
PRF1 (perforin)	0.1	0	0	0	41	15	274	632
GNLY (Granulycin)	0.7	0.8	6	2	109	50	722	205
FOXP3	0	0.3	0	0.1	30	23	2	0.2
GATA3	0.1	0.4	0.4	0	196	174	150	281
TBX21 (T-bet)	5	3	0	0	27	15	78	21
CCR7	83	52	0	0	124	129	145	286
CD44	4	2	4	4	3	4	2	14
SPN (CD43)	0	0	0.9	0.7	20	10	52	29
KLRG1	3	4	0.4	0.9	101	97	197	178
NKG2D(KLRK1)	34	19	0.3	0	5	0.4	641	424
NCAM1 (CD56)	0	0.2	3	6	0.7	2	17	21
KLRB1 (NK1.1)	0.2	0.3	0.5	0	504	245	420	183

CD19⁺ B-cells, CD14⁺ monocytes and both CD4⁺ and CD8⁺ T cells were analyzed for their expression of a panel of T cell granule proteins and cell surface markers and transcription factors of T cells and other immune cells including the granzymes, perforin, granulycin, FOXP3, GATA3, T-bet (TBX21), CCR7, CD44, CD43, KLRG1, KLRK1, NCAM1(CD56) and KLRB1 (NK1.1). The number of reads, which directly correspond to their expression level, is presented for two individual samples originating from two different blood donors for each cell population. Among these four cell populations only the T cells express these granule proteins. Compared to lysozyme expression in monocytes (approximately 20 000 reads), the expression level of granzymes, perforin and granulysin, in T cells are relatively low.

The bold is for values of 10 or higher to highlight the highly expressing cells.